Atract™

Name: Atract™ Kit
Brand: Bio-ReCell
Price: 1.0 EUR
Availability: InStock

Atract™ Kit is our T-cell activation reagent, consisting of biocompatible, metal-free particles with irreversibly bound anti-CD3, anti-CD28 and anti-CD2 antibodies, designed to interact with naive T lymphocytes and trigger their activation and expansion. We offer an improved version of the product, Atract™ Kit V2, which is further optimised for superior proliferation and a more user-friendly workflow.

The Atract™ Kit reagent was designed with our slogan and guidance in mind: “No magnets, no residuals just pure cells”. Reagent particles cannot be phagocytised and are easy to remove from the cell culture. Atract™ Kit is compatible with and scalable across conventional culture flasks, bags, plates and G-Rex platforms.

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Atract™ Kit is offered in two different formats, adapted to different applications and scales of T-cell research. We are happy to advise and try to help tailor your Atract™ Kit experience to your unique needs, as well as provide you with additional knowledge and tips, based on years of research, development and optimization of our activation reagent.

Atract™ Kit V2 (50 mg)

Anti-CD2/-CD3/-CD28

Contents:
4x 50 mg of Bio-ReCell CD2/CD3/CD28 biocompatible particles

Contents of the kit are sufficient to activate up to 60 million peripheral blood mononuclear cells (PBMCs).

Atract™ Kit V2 (10 mg)

Anti-CD2/-CD3/-CD28

Contents:
4x 10 mg of Bio-ReCell CD2/CD3/CD28 biocompatible particles

Contents of the kit are sufficient to activate up to 12 million peripheral blood mononuclear cells (PBMCs).

For more information about the performance of the Atract™ Kits, download our information brochure.

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Why Atract™

Rapid activation with strong proliferation and expansion.

Clean cell product, free of magnets and impurities.

Robust functionality and scalability across different platforms.

Data

Comparison of T-cell count between the Atract™ Kit and a market competitor after nine days of expansion in various scales of the G-Rex platform.

Proportions of T-cell subtypes for the Atract™ Kit and a market competitor product after 48h of activation.

FAQ

Which samples are appropriate for activation with Atract™?

Peripheral blood mononuclear cells (PBMCs), apheresis samples, and purified T cells have been tested, and the results were comparable.

Is Atract™ reagent removal necessary?

No, but it can be easily removed at any step of the process. Atract™ has demonstrated non-toxic characteristics, but it may affect subsequent steps in the process.

Which 20-35 μm strainers are suitable for filtering the suspension?

We offer an Atract™ removal kit, which is suitable for use with G-Rex®6M Well Plates. For other formats, there are also many commercially available filter tubes and strainers that are suitable for filtering the desired amount of suspension.

Can we use different protocols and adjust the official protocol?

Atract™ has been extensively tested with various formats, including cell culture-treated wells, G-Rex containers, and cell culture bags. The proposed cell concentration and Atract™ amounts resulted in higher expansion and viability in our studies. However, the protocol can be adjusted for your specific needs.

Are there any requirements for cell culture containers?

Atract™ does not require adherent surfaces. Achieving a uniform distribution of Atract™ is essential, and careful pipetting is crucial due to its quick sedimentation.

Why does the protocol suggest adjustments for 96-, 48-, 24-, and 12-well plates?

This ensures accurately prepared aliquots of Atract™ and uniform distribution across the wells.

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Atract™

FAQ

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